![]() ![]() Select keep source images to keep original images open. Determine which image will be which channel (red, green, blue, cyan etc.) and merge using the dropdown menu. To merge several images, go to Image > Color > Merge Channels.To pseudocolor the image, go to Image > LookUp Table and select desired color.To change your image, go to Image > Type and select desired type.Ĭolor Processing: Make sure the selected channel or image is the primary window. Since this creates an overlay of the scale bar, you need to combine your image and the overlay by going to Image > Overlay > Flatten.Ĭhanging image type: Some microscopes create a higher quality 16-bit image, but 8-bit images work best to allow you to view them on your computer without any analysis software. To open a Z-stacked image, ensure you are splitting channels if a multi-channeled Z-stack.Īdding a scale bar: Go to Analyze > Tools > Scalebar.Ensure that the channels are colored correctly, as Fiji can occasionally misread the metadata. You can re-merge the channels later if desired. Splitting channels allows you to alter the channel color, if necessary. To open a multi-channel image, open your image the same way as a single channel and choose to “Split Channels”.Ensure the image is opened as a Hyperstack.If the image is a life science image file, the bio-formats window will open. ![]()
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